45·107 (2.37·106) DH(KCALÆMOL)1))23.21(0.23))0.82(0.36))10.55(0.16))...

1.45·10

7

(2.37·10

6

) DH(kcalÆmol

)

1

))23.21(0.23))0.82(0.36))10.55(0.16))11.94(0.14))13.9(0.24))14.8(0.25))27.0(0.40) TDS(kcalÆmol

)

1

)[DH–DG])13.52(0.42)5.32(0.53))0.68(0.19))4.03(0.14))5.72(0.24))6.86(0.25))17.4(0.41) DG

bind

ITC(kcalÆmol

)

1

))9.69(0.26))6.14(0.39))9.87(0.11))7.91(0.03))8.18(0.04))7.94(0.03))9.60(0.10) K

D

20CITC(

M

)

1

thermal)60n

M

(25C)(N⁄A)26.6l

M

(324l

M)

45n

M

(4n

M)

1.27l

M

(20l

M)

0.79l

M

(40l

M)

1.2l

M

(0.45l

M)

69n

M

(0.15n

M)

DGthermal(kcalÆmol

)

1

)

A

N⁄A)5.5)11.4)8.6)8.1)8.5)18.0 MeasuredT

m

N⁄A166345444498

a

bCIPAT

m

b

25136256374990 BOW

c

N⁄A26.941.435.133.433.455.6

a

Extrapolatedfromfitusingarestrainedupperbaselinebasedonalternativedimerthermaldenaturationprofiles.

b

Predictedthermalmeltingvaluebasedonsequencedatausingbasic coiledcoilinteractionalgorithm(bCIPA).

c

Predictedinteractionscoreaccordingtobaseoptimizedweights(BOWs)[29].Agadir (293 K)helical (ƒ

_H

)(150l

M

Pt) h

₂₂₂

⁄h

₂₀₈

cJun 0.53 14.6 3.7cFos 0.65 17.3 3.5FosW 1.02 43.7 26.2JunW 1.01 41.7 17.0JunW

_CANDI

0.79 22.2 21.9FosW

Core

0.74 26.6 10.2