1 STOICHIOMETRY) TO EXTRACT THE VARIOUS THERMODYNAMICTEIN-FRAGMENT COM...

1 : 1 stoichiometry) to extract the various thermodynamic

tein-fragment complementation assays. Proc Natl Acad

parameters [18], namely the apparent equilibrium constant

Sci USA

(K

d

) and the enthalpy change (DH) associated with hetero-

11 Mason JM, Schmitz MA, Muller KM & Arndt KM

dimerization. The free energy change (DG

_bind

) upon ligand

(2006) Semirational design of Jun–Fos coiled coils with

binding can be calculated from the relationship:

increased affinity: universal implications for leucine

bind

ln

D

zipper prediction and design. Proc Natl Acad Sci USA

where R is the universal molar gas constant (1.9872 kcalÆ